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KMID : 1023420090090020249
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Journal of Dental Hygiene Science
2009 Volume.9 No. 2 p.249 ~ p.255
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Comparison between Bacterial Culture Method and Multiplex PCR for Identification of Fusobacterium nucleatum and Actinobacillus actinomycetemcomitans from the Dental Plaques
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Kim Hwa-Sook
Lim Sun-A
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Abstract
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This study was carried out for the purpose of comparing bacterial culture method, single PCR, and multiplex PCR for identification of F. nucleatum and A. actinomycetemcomitans in subgingival plaque of adult periodontitis. Targeting 20 patients with adult periodontitis, the subgingival plaque was collected in teeth, respectively, for #16, #36, #44. A bacillus was cultivated by painting it over the solid selective media of F. nucleatum and A. actinomycetemcomitans. Bacterial species were detected in 0 tooth with 12 pieces, respectively. Through single PCR and multiplex PCR, the positive reaction was indicated in 43 teeth with 45 pieces, respectively, as for F. nucleatum, and in 1 tooth with 4 pieces, respectively, as for A. actinomycetemcomitans. In the comparative analysis between bacterial identification methods, F. nucleatum showed the more statistically significant difference(p=0.000) in comparison between single PCR and multiplex PCR. Even A. actinomycetemcomitans was indicated significantly(p=0.067) in a case that is based on 0.1 in significant level in the comparison between single PCR and multiplex PCR. In conclusion, as a result of comparing the bacterial identification methods, the detection frequency was indicated to be higher in PCR than in bacterial culture method. Single PCR and multiplex PCR showed the mutually similar detection frequency. Accordingly, given thinking of economic efficiency, quickness, and reduction in labor force, it is thought to be more efficient method to use single PCR as the bacterial identification method.
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KEYWORD
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Fusobacterium nucleatum, Actinobacillus actinomycetemcomitans, Culture method, Multiplex PCR, Single PCR
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